A TRAF and TNF receptor-associated protein (TTRAP) in mollusk with endonuclease activity

doi:10.1016/j.dci.2011.02.013

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	A TRAF and TNF receptor-associated protein (TTRAP) in mollusk with endonuclease activity
	Yang, Jialong 1,2; Qiu, Limei 1; Wang, Leilei 1,2; Huang, Mengmeng1,2 ; Wang, Lingling 1; Zhang, Huan 1; Song, Linsheng 1
	2011-08-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	35 期号:8 页码:827-834
文章类型	Article
摘要	Tumor necrosis factor (TNF) signaling pathway plays crucial roles in the regulation of various immune responses. In the present study, a TNF signaling pathway related regulatory factor, TRAF and TNF receptor-associated protein (MAP), was firstly identified from the mollusk Zhikong scallop Chlamys farreri (designated as CfTTRAP). The full-length cDNA of CfTTRAP was of 2326 bp, containing an open reading frame (ORF) of 1008 bp encoding a polypeptide of 335 amino acids with the predicted molecular weight of 38.4 kDa. There was an Exo_endo_phos domain in CfTTRAP, and it was well conserved when compared with other TTRAPs, especially the endonuclease activity related motifs. The recombinant protein of CM-RAP exhibited prominent endonuclease activity to digest the genome DNA from C. farreri in the presence of Mg2+, but it could not digest genome DNA of Escherichia coli and Bacillus subtilis, indicating CfTTRAP was a new member of Mg2+/Mn2+-dependent phosphodiesterase enzymes (MOP) superfamily. The mRNA transcripts of CfTTRAP were detected in all tested tissues of scallop, including muscle, mantle, gonad, gill, kidney and hemocytes. The expression level of CfTTRAP mRNA in hemocytes varied greatly after the stimulation of LPS, PGN or beta-glucan. LPS induced significant down-regulation (P<0.05) of CfTTRAP mRNA expression, while PGN or beta-glucan up-regulated the expression significantly (P<0.01), indicating that this regulatory factor was involved in modulating immune responses towards different stimulus. The present results provided new evidences for the potential roles of such molecule in C. farreri, and further confirmed the existence of TTRAP modulated TNF signaling pathway in mollusk. (C) 2011 Elsevier Ltd. All rights reserved.; Tumor necrosis factor (TNF) signaling pathway plays crucial roles in the regulation of various immune responses. In the present study, a TNF signaling pathway related regulatory factor, TRAF and TNF receptor-associated protein (MAP), was firstly identified from the mollusk Zhikong scallop Chlamys farreri (designated as CfTTRAP). The full-length cDNA of CfTTRAP was of 2326 bp, containing an open reading frame (ORF) of 1008 bp encoding a polypeptide of 335 amino acids with the predicted molecular weight of 38.4 kDa. There was an Exo_endo_phos domain in CfTTRAP, and it was well conserved when compared with other TTRAPs, especially the endonuclease activity related motifs. The recombinant protein of CM-RAP exhibited prominent endonuclease activity to digest the genome DNA from C. farreri in the presence of Mg(2+), but it could not digest genome DNA of Escherichia coil and Bacillus subtilis, indicating CfTTRAP was a new member of Mg(2+)/Mn(2+)-dependent phosphodiesterase enzymes (MOP) superfamily. The mRNA transcripts of CfTTRAP were detected in all tested tissues of scallop, including muscle, mantle, gonad, gill, kidney and hemocytes. The expression level of CfTTRAP mRNA in hemocytes varied greatly after the stimulation of LPS, PGN or beta-glucan. LPS induced significant down-regulation (P<0.05) of CfTTRAP mRNA expression, while PGN or beta-glucan up-regulated the expression significantly (P<0.01), indicating that this regulatory factor was involved in modulating immune responses towards different stimulus. The present results provided new evidences for the potential roles of such molecule in C. farreri, and further confirmed the existence of TTRAP modulated TNF signaling pathway in mollusk. (C) 2011 Elsevier Ltd. All rights reserved.
关键词	Chlamys Farreri Ttrap Innate Immunity Tnf Receptor Signaling Pathway Endonuclease Activity
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2011.02.013
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000292441000003
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11767
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Yang, Jialong,Qiu, Limei,Wang, Leilei,et al. A TRAF and TNF receptor-associated protein (TTRAP) in mollusk with endonuclease activity[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2011,35(8):827-834.
APA	Yang, Jialong.,Qiu, Limei.,Wang, Leilei.,Huang, Mengmeng.,Wang, Lingling.,...&Song, Linsheng.(2011).A TRAF and TNF receptor-associated protein (TTRAP) in mollusk with endonuclease activity.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,35(8),827-834.
MLA	Yang, Jialong,et al."A TRAF and TNF receptor-associated protein (TTRAP) in mollusk with endonuclease activity".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 35.8(2011):827-834.

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