Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| A multivalent killed whole-cell vaccine induces effective protection against Edwardsiella tarda and Vibrio anguillarum | |
| Sun, Yun1,3; Liu, Chun-sheng2; Sun, Li1 | |
| 2011-10-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 31期号:4页码:595-599 |
| 文章类型 | Article |
| 摘要 | Edwardsiella tarda, Vibrio anguillarum, Streptococcus iniae, and Vibrio harveyi are important aquaculture pathogens that affect a wide range of fish species. In this study, we examined in a Japanese founder (Paralichthys olivaceus) model the possibility of developing simple, killed whole-cell vaccines with protective effect against two or more of the above pathogens. For this purpose, we utilized four pathogenic fish isolates: E. tarda TX1, V. anguillarum C312, S. iniae SF1, and V. harveyi T4D. Flounder were immunized with inactivated monovalent TX1, C312, SF1, or T4D, or with different combinations of these strains in the form of (i): TX1, C312, SF1, and T4D mix (M4); (ii)TX1, C312, and SF1 mix (M3); (iii)TX1 and 012 mix (M2). Fish were challenged at two months post-vaccination with E. tarda, V. anguillarum, S. iniae, or V. harveyi and monitored for mortality. The results showed that the monovalent vaccines produced low protections, with relative percent survival (RPS) rates between 33.3% and 53.9%. Compared to monovalent vaccines, M2, M3, and M4 all induced significantly higher levels of protection against E. tarda. In addition, M2 and M4 also effected much higher (similar to 19%) RPS rates against V. anguillarum. Serum antibody production and bactericidal activity analysis showed a correlation between protection efficacy and antibody level. These results suggest a humoral immunity-based mechanism of protection induced by inactivated whole-cell vaccines, and that there exists a mutual and specific immunostimulatory effect between E. tarda TX1 and V. anguillarum C312, which enables the divalent M2 to induce effective protective immunity against E. tarda and V. anguillarum. (C) 2011 Elsevier Ltd. All rights reserved.; Edwardsiella tarda, Vibrio anguillarum, Streptococcus iniae, and Vibrio harveyi are important aquaculture pathogens that affect a wide range of fish species. In this study, we examined in a Japanese founder (Paralichthys olivaceus) model the possibility of developing simple, killed whole-cell vaccines with protective effect against two or more of the above pathogens. For this purpose, we utilized four pathogenic fish isolates: E. tarda TX1, V. anguillarum C312, S. iniae SF1, and V. harveyi T4D. Flounder were immunized with inactivated monovalent TX1, C312, SF1, or T4D, or with different combinations of these strains in the form of (i): TX1, C312, SF1, and T4D mix (M4); (ii)TX1, C312, and SF1 mix (M3); (iii)TX1 and 012 mix (M2). Fish were challenged at two months post-vaccination with E. tarda, V. anguillarum, S. iniae, or V. harveyi and monitored for mortality. The results showed that the monovalent vaccines produced low protections, with relative percent survival (RPS) rates between 33.3% and 53.9%. Compared to monovalent vaccines, M2, M3, and M4 all induced significantly higher levels of protection against E. tarda. In addition, M2 and M4 also effected much higher (similar to 19%) RPS rates against V. anguillarum. Serum antibody production and bactericidal activity analysis showed a correlation between protection efficacy and antibody level. These results suggest a humoral immunity-based mechanism of protection induced by inactivated whole-cell vaccines, and that there exists a mutual and specific immunostimulatory effect between E. tarda TX1 and V. anguillarum C312, which enables the divalent M2 to induce effective protective immunity against E. tarda and V. anguillarum. (C) 2011 Elsevier Ltd. All rights reserved. |
| 关键词 | Edwardsiella Tarda Streptococcus Iniae Vibrio Anguillarum Vibrio Harveyi Vaccine |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2011.06.025 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000295020000011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/11751 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Sun, Yun,Liu, Chun-sheng,Sun, Li. A multivalent killed whole-cell vaccine induces effective protection against Edwardsiella tarda and Vibrio anguillarum[J]. FISH & SHELLFISH IMMUNOLOGY,2011,31(4):595-599. |
| APA | Sun, Yun,Liu, Chun-sheng,&Sun, Li.(2011).A multivalent killed whole-cell vaccine induces effective protection against Edwardsiella tarda and Vibrio anguillarum.FISH & SHELLFISH IMMUNOLOGY,31(4),595-599. |
| MLA | Sun, Yun,et al."A multivalent killed whole-cell vaccine induces effective protection against Edwardsiella tarda and Vibrio anguillarum".FISH & SHELLFISH IMMUNOLOGY 31.4(2011):595-599. |
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