Toxic effects of Karenia mikimotoi extracts on mammalian cells

doi:10.1007/s00343-011-0514-8

	Toxic effects of Karenia mikimotoi extracts on mammalian cells
	Chen Yang; Yan Tian; Yu Rencheng; Zhou Mingjiang
	2011-07-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	29 期号:4 页码:860-868
文章类型	Article
摘要	Karenia is one of the most harmful and representative red tide genus in a temperate zone. Blooms caused by this genus have resulted in massive fish death in the South China Sea and the East China Sea. However, the potential effects of this dinoflagellate on human health through the transfer of toxins via marine food webs, and the mechanisms of toxicity, are still unknown. Therefore, we examined the toxic effects of a strain of K. mikimotoi (isolated from the South China Sea) on the proliferation and morphology of four mammalian cell lines (two normal cell lines and two cancer cell lines). In addition, we carried out a preliminary investigation on the mechanism of toxicity of the alga. The results show that the polar lipid-soluble component of K. mikimotoi significantly inhibited proliferation of the four cell lines, and resulted in the cells becoming spherical, swollen and damaged. The result of Annexin V and PI double-staining confirmed that cell membranes were disrupted. The malonaldehyde (MDA) contents in the medium of the four cell lines treated with the polar-lipid extracts all increased significantly, which indicates that the polar-lipid toxins produced by K. mikimotoi could adversely affect mammalian cells by inducing lipid peroxidation. We conclude that K. mikimotoi is a potential threat to human health, and the comprehensive effect of this dinoflagellate and its mechanisms should be investigated further.; Karenia is one of the most harmful and representative red tide genus in a temperate zone. Blooms caused by this genus have resulted in massive fish death in the South China Sea and the East China Sea. However, the potential effects of this dinoflagellate on human health through the transfer of toxins via marine food webs, and the mechanisms of toxicity, are still unknown. Therefore, we examined the toxic effects of a strain of K. mikimotoi (isolated from the South China Sea) on the proliferation and morphology of four mammalian cell lines (two normal cell lines and two cancer cell lines). In addition, we carried out a preliminary investigation on the mechanism of toxicity of the alga. The results show that the polar lipid-soluble component of K. mikimotoi significantly inhibited proliferation of the four cell lines, and resulted in the cells becoming spherical, swollen and damaged. The result of Annexin V and PI double-staining confirmed that cell membranes were disrupted. The malonaldehyde (MDA) contents in the medium of the four cell lines treated with the polar-lipid extracts all increased significantly, which indicates that the polar-lipid toxins produced by K. mikimotoi could adversely affect mammalian cells by inducing lipid peroxidation. We conclude that K. mikimotoi is a potential threat to human health, and the comprehensive effect of this dinoflagellate and its mechanisms should be investigated further.
关键词	Karenia Mikimotoi Mammalian Cells Super-oxidation
学科领域	Marine & Freshwater Biology ; Oceanography
DOI	10.1007/s00343-011-0514-8
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000292558700014
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11725
专题	海洋生态与环境科学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Chen Yang,Yan Tian,Yu Rencheng,et al. Toxic effects of Karenia mikimotoi extracts on mammalian cells[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2011,29(4):860-868.
APA	Chen Yang,Yan Tian,Yu Rencheng,&Zhou Mingjiang.(2011).Toxic effects of Karenia mikimotoi extracts on mammalian cells.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,29(4),860-868.
MLA	Chen Yang,et al."Toxic effects of Karenia mikimotoi extracts on mammalian cells".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 29.4(2011):860-868.

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