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半滑舌鳎 CC 型趋化因子和 SOCS 的系统发育和表达模式分析
郝连旭
Subtype硕士
Thesis Advisor孙黎
2016-05-19
Degree Grantor中国科学院大学
Place of Conferral北京
Degree Discipline海洋生物学
Keyword半滑舌鳎、cc型趋化因子、socs、抗细菌免疫、免疫防御
Other Abstract
       本研究以半滑舌鳎(Cynoglossus semilaevis)为研究对象,分析了其9个CC型趋化因子(CC chemokine,CCL)和6个细胞因子信号传导抑制因子(suppressor of cytokine signaling,SOCS)的蛋白序列特征及系统发育,研究了这些因子在不同条件下的基因表达模式。
      CC型趋化因子是一类广泛存在的小分子细胞因子,它们能够趋化白细胞向宿主体内炎症部位迁移。半滑舌鳎作为我国特有的养殖品种,经济价值巨大。本研究构建了半滑舌鳎和其它脊椎动物的CC型趋化因子的系统进化树。根据舌鳎9个CC型趋化因子所处的系统进化位置,确定了它们的命名和分类。在正常生理条件下,大部分趋化因子在头肾、鳃和肠道等免疫器官中的mRNA表达水平较高。在病原微生物感染条件下,不同趋化因子的mRNA表达具有组织特异性和时间特异性,CsCCL20a和CsCCL21在细菌感染后的表达水平急速地显著上升,暗示它们作为炎症型趋化因子在先天性免疫反应中可能发挥重要作用。相对而言,CsCCL20b和CsCCL27b在细菌感染后表达无显著变化,表明它们可能在维持机体内稳态方面发挥作用。这些结果说明这些CC型趋化因子可能作为炎症型或内稳态型趋化因子在半滑舌鳎的先天性免疫中发挥作用。
      SOCS是细胞因子信号通路的负调控因子,通过负反馈抑制细胞因子的信号传递,防止过量的信号反应干扰正常的代谢平衡和细胞功能。SOCS在哺乳动物中的研究相对深入,而在硬骨鱼类中的研究相对匮乏。在本研究中,我们发现6个半滑舌鳎SOCS(命名为CsSOCS)具有保守的SOCS结构域,而且系统发育分析将它们与哺乳动物和其它硬骨鱼中已知同源的SOCS归为一类。在正常生理条件下,除CsSOCS2之外,其它CsSOCS在肾脏中的mRNA表达量最高,在鳃和脾脏中表达也相对较高。在不同病原感染情况下,6个CsSOCS的表达水平不同,表明CsSOCS的表达具有时间、组织和病原特异性。这些结果暗示不同CsSOCS在半滑舌鳎应对病原感染时发挥不同作用。

;       In this study, we have identified and characterized nine CC chemokines (CCL) and six suppressors of cytokine signaling (SOCS) from half smooth tongue sole (Cynoglossus semilaevis). We also performed their tissue specific expression patterns under normal physiological conditions and their expression profiles in response to the challenge of Vibrio harveyi, Edwardsiella tarda and megalocytivirus RBIV-C1.
      CC chemokines are a large, diverse group of small cytokines plays a pivotal role in host defense by inducing leukocyte chemotaxis under physiological and inflammatory conditions. Tongue sole is a species of great economic importance in aquaculture. In this study, we named these chemokines according to the results of the phylogenetic tree, which showed that the nine CCLs distributed in different phylogenetic groups. The tissue specific expression patterns of the 9 chemokines under normal physiological conditions varied much, with most chemokines highly expressed in immune organs, such as head kidney, gill and intestine. In addition, the 9 chemokines exhibited similar or distinctly different expression profiles in a tissue-specific and time-dependent manner. The expression level of CsCCL20a and CsCCL21 were extremely high, which may serve as gene markers linked to antibacterial immunity in tongue sole. These results suggest they are inflammatory chemokines that react quickly to pathogen invasion. Meanwhile, CsCCL20b and CsCCL27a showed weak reactions to bacterial and viral pathogen, suggesting that they are likely homeostatic chemokines. These results indicate that in tongue sole, CC chemokines may be involved in different immune responses as homeostatic or inflammatory chemokines.
      Suppressors of cytokine signaling (SOCS) family is a group of important feedback inhibitors of cytokine receptor signaling in case that excessive cytokine signaling disrupt the normal homeostasis and cellular functions. They have been extensively studied in mammalian models, but systematic analyses of SOCS in teleost species are limited. In the current study, all six SOCS genes (CsSOCS2 to CsSOCS7) from tongue sole (Cynoglossus semilaevis) exhibit conserved structures of SOCS and were phylogenetically grouped together with the respective SCOS members known in mammalian and teleost species. Under normal physiological conditions, the expression of the six CsSOCS genes were detected at varied levels in nine major tissues, with most CsSOCS highly expressed in head kidney, gill and spleen. Most CsSOCS genes exhibited distinctly different expression profiles in a time-, tissue-, and pathogen-dependent manner. These results indicate that the same bacterial pathogen induces different expression patterns for the six CsSOCS genes, suggesting that different members of SOCS may play different roles in tongue sole. In general, E. tarda enhanced the expressions of CsSOCS in more tissues and with higher induction folds than V. harveyi. It is possible that the augmented expressions of these SCOS members in fish tissues may facilitate bacterial invasion by inhibiting the expression of cytokines with antibacterial functions.
Subject Area地球科学 ; 海洋科学 ; 海洋生物学
Language中文
Document Type学位论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/117011
Collection实验海洋生物学重点实验室
Affiliation中国科学院海洋研究所
Recommended Citation
GB/T 7714
郝连旭. 半滑舌鳎 CC 型趋化因子和 SOCS 的系统发育和表达模式分析[D]. 北京. 中国科学院大学,2016.
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