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中国海洋红藻分子系统发育初步分析及石花菜目的分类学研究
Alternative Title
王旭雷
Subtype博士
Thesis Advisor王广策 ; 夏邦美
2016-05
Degree Grantor中国科学院大学
Place of Conferral北京
Degree Discipline海洋生物学
Keyword红藻门 分子系统 形态分类 石花菜目 新种
Abstract红藻门中的很多类群都是经济海藻,在海藻工业、海藻化学、水产养殖中发挥着重要的作用。《中国海藻志》的陆续出版表明我国海藻传统分类学研究有着深厚的研究基础。近年来藻类分子系统学的研究开启了藻类分类学的另一扇大门,并取得了一系列重要的成果。然而,我国海藻分子系统学的研究基础却非常薄弱。为此,作者进行了多次野外红藻资源调查,样品采集点覆盖了我国四大海藻区系,采集样品1038号,共有133个物种成功扩增序列617条;其中,rbcL序列318条,分布在15目、30科、46属、91个物种(包括未确定种名的物种21个),COI序列299条,分布在13目、28科、50属、101个物种(包括未确定种名的物种25个);这些序列为我国红藻分子系统学的研究提供了基础数据,也为全球其它相关海藻区系的红藻分子系统学研究提供了重要的参考资料。同时,揭示了11个分类单元将来可能描述为我国的新纪录种或新种。通过48个物种97个样品的rbcL+COI组合序列构建了我国红藻分子系统发育树,初步分析了我国海洋红藻类群目、科级的系统发育关系,从分子生物学的视角初步反映了我国海洋红藻系统的面貌,并由此跟随国际上藻类分类系统的变化,修订了8个分类单元。
在以上分子系统发育分析的基础上,针对重要经济海藻石花菜目类群,系统的开展了形态学和分子系统发育学研究,分析了我国石花菜科、鸡毛菜科的种属关系。新增了我国石花菜目1个新记录属:阿凡达藻属Aphanta Tronchin & Freshwater,描述了4个新种,分别是红海湾石花菜Gelidium honghaiwanense G. C. Wang et X. L. Wang sp. nov.、杨梅坑石花菜Gelidium yangmeikengense G. C. Wang et X. L. Wang sp. nov.、三亚石花菜 Gelidium sanyaense G. C. Wang et X. L. Wang sp. nov.和亚洲阿凡达藻Aphanta asiatica G. C. Wang et X. L. Wang sp. nov.;修订Gelidium tsengiiGelidium johnstonii的同物异名;分析了细毛石花菜类群形态变异特征。通过构建我国石花菜目的分子系统进化树,揭示我国石花菜目类群应为3个科5个属,并在此基础上修订了我国石花菜目类群的分科检索表和分属检索表。
Other AbstractMany groups of Rhodophyta are economically important which play an important role in the seaweed industry, seaweed chemistry and aquaculture. Chinese traditional seaweed taxonomy has been well studied as a series of publishing of monographs. Recent studies of algal molecular systematics shed new sights in the traditional algal taxonomy, and achieved a lot of important results. However, the molecular phylogenetic study of Chinese rhodophyta is very weak.
In this paper, We conducted a number of Rhodophyta biodiversity survey along Chinese coast, with a sampling collection sites coverage of China's four major algal floras. More than 1,000 samples were collected, and 617 sequences were successfully amplified, of which 318 rbcL sequences and 299 COI sequences were generated. The rbcL sequences distributed in 15 orders, 30 families, 46 genera and 91 species (including 21 undetermined species), and COI distributed in 13 orders, 28 families, 50 genera and 101 species (including 25 undetermined species). These sequences of this study provide the baseline data for the future molecular phylogenetic studies of Chinese marine red algae or even other related marine algal flora. At the same time, 11 newly discovered taxa or new records for the Chinese marine algal flora are in progress for description. Phylogenetic analysis of 98 concatenated rbcL and COI sequences among 48 species revealed a tree that resembled the current recognized florideophyte red algal relationships, and thus 8 taxa names are updated accordingly.
Based upon morphological and molecular data analysis, a systematical study of the order Gelidiales (Rhodophyta) from China are conducted. The results revealed  the phylogenetic relationships between the Gelidiaceae and Pterocladiaceae species. One new genus Aphanta Tronchin & Freshwater is added and four species are newly described, namely Gelidium honghaiwanense G. C. Wang et X. L. Wang sp. nov., Gelidium yangmeikengense G. C. Wang et X. L. Wang sp. nov., Gelidium sanyaense G. C. Wang et X. L. Wang sp. nov. and Aphanta asiatica G. C. Wang et X. L. Wang sp. nov.; Gelidium tsengii was revised as a synonym of Gelidium johnstonii; the morphological variations of Gelidium crinale has been discussed. Based upon the above data, a phylogenetic analysis of Chinese order Gelidiales revealed a tree that actually represent 3 families and 5 genera.
Subject Area地球科学 ; 海洋科学 ; 海洋生物学
Language中文
Document Type学位论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/116961
Collection实验海洋生物学重点实验室
Affiliation中国科学院海洋研究所
Recommended Citation
GB/T 7714
王旭雷. 中国海洋红藻分子系统发育初步分析及石花菜目的分类学研究[D]. 北京. 中国科学院大学,2016.
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