Graphene Oxide Sheet-Mediated Silver Enhancement for Application to Electrochemical Biosensors

doi:10.1021/ac103047c

	Graphene Oxide Sheet-Mediated Silver Enhancement for Application to Electrochemical Biosensors
	Wan, Yi 1,2; Wang, Yi1 ; Wu, Jiajia1,2 ; Zhag, Dun 1; Zhag, D (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Corros Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
	2011-02-01
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
卷号	83 期号:3 页码:648-653
文章类型	Article
摘要	Functionalized graphene oxide (GO) sheets coupled with a signal amplification method based on the nanomaterial-promoted reduction of silver ions for the sensitive and selective detection of bacteria. This paper aims to develop an electrochemical route combined with GO sheet-mediated Ag enhancement for biological/chemical analyte detection. A linear relationship between the stripping response and the logarithm of the bacterial concentration was obtained using an electrochemical technique for concentrations ranging from 1.8 x 10(2) to 1.8 x 10(8) cfu mL(-1), with a slope of 15.28 and a correlation coefficient of 0.995. Dot blot assay was used as a conventional immunoassay method for comparison with the electrochemical method, as well as to observe the quality of the anti-sulfite-reducing bacteria (SRB) antibody (Ab) used in the immunosensor. The GO sheet-mediated,silver enhancement holds great potential for the rapid analysis of protein, DNA, and pathogens.; Functionalized graphene oxide (GO) sheets coupled with a signal amplification method based on the nanomaterial-promoted reduction of silver ions for the sensitive and selective detection of bacteria. This paper aims to develop an electrochemical route combined with GO sheet-mediated Ag enhancement for biological/chemical analyte detection. A linear relationship between the stripping response and the logarithm of the bacterial concentration was obtained using an electrochemical technique for concentrations ranging from 1.8 x 10(2) to 1.8 x 10(8) cfu mL(-1), with a slope of 15.28 and a correlation coefficient of 0.995. Dot blot assay was used as a conventional immunoassay method for comparison with the electrochemical method, as well as to observe the quality of the anti-sulfite-reducing bacteria (SRB) antibody (Ab) used in the immunosensor. The GO sheet-mediated,silver enhancement holds great potential for the rapid analysis of protein, DNA, and pathogens.
学科领域	Chemistry
DOI	10.1021/ac103047c
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000286689600004
引用统计	被引频次：161[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11449
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Zhag, D (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Corros Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Shandong Prov Key Lab Corros Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wan, Yi,Wang, Yi,Wu, Jiajia,et al. Graphene Oxide Sheet-Mediated Silver Enhancement for Application to Electrochemical Biosensors[J]. ANALYTICAL CHEMISTRY,2011,83(3):648-653.
APA	Wan, Yi,Wang, Yi,Wu, Jiajia,Zhag, Dun,&Zhag, D .(2011).Graphene Oxide Sheet-Mediated Silver Enhancement for Application to Electrochemical Biosensors.ANALYTICAL CHEMISTRY,83(3),648-653.
MLA	Wan, Yi,et al."Graphene Oxide Sheet-Mediated Silver Enhancement for Application to Electrochemical Biosensors".ANALYTICAL CHEMISTRY 83.3(2011):648-653.

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