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The immunological capacity in the larvae of Pacific oyster Crassostrea gigas
Song, Xiaorui1,3; Wang, Hao1; Xin, Lusheng1,3; Xu, Jiachao1,3; Jia, Zhihao1,3; Wang, Lingling1; Song, Linsheng1,2
2016-02-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume49Pages:461-469
SubtypeArticle
AbstractAs the immune system has not fully developed during early developmental stages, bivalve larvae are more susceptible for pathogens, which frequently leads to the significant mortality in hatcheries. In the present study, the development of immune system and its response against bacteria challenge were investigated in order to characterize the repertoire of immunological capacity of Pacific oyster Crassostrea gigas during the ontogenesis. The phagocytosis was firstly observed in the early D-veliger larvae (17 hpf), especially in their velum site, which indicated the appearance of functional hemocytes during early Dveliger larvae stage. The whole-mount immunofluorescence assay of three pattern recognition receptors (integrin beta-1, caspase-3 and C-type lectin 3) and one immune effector gene (IL17-5) was performed in blastula, early D-veliger and umbo larvae, suggested that velum and digestive gland were the potential sites of immune system in the larvae. The lowest activities of antioxidant enzymes (superoxide dismutase and catalase) and hydrolytic enzyme (lysozyme), as well as descended expression levels of 12 immune genes at the transition between embryogenesis and planktonic, indicated that the larvae at hatching (9 hpf) were in hypo-immunity. While the ascending activities of enzymes and expression levels of seven immune genes during the trochophore stage (15 hpf) suggested the initiation of immune system. The steadily increasing trend of all the 12 candidate genes at the early umbo larvae (120 h) hinted that the immune system was well developed at this stage. After bacterial challenge, some immune recognition (TLR4) and immune effector (1L17-5 and defh2) genes were activated in blastula stage (4 hpf), and other immune genes were up regulated in D-veliger larvae, indicating that the zygotic immune system could respond earlier against the bacterial challenge during its development. These results indicated that the cellular and humoral immune components appeared at trochophore stage, and the cellular immune system was activated with its occurrence, while the humoral immune system executed until the early umbo larval stage. The immune system emerged earlier to aid larvae in defending bacterial challenge during the early stages of oyster development. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordPacific Oyster Crassostrea Gigas Phagocytosis Immune System Hatching Trochophore Stage Umbo Larvae
DOI10.1016/j.fsi.2016.01.009
Indexed BySCI
Language英语
WOS IDWOS:000370884200050
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/112487
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Song, Xiaorui,Wang, Hao,Xin, Lusheng,et al. The immunological capacity in the larvae of Pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,49:461-469.
APA Song, Xiaorui.,Wang, Hao.,Xin, Lusheng.,Xu, Jiachao.,Jia, Zhihao.,...&Song, Linsheng.(2016).The immunological capacity in the larvae of Pacific oyster Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,49,461-469.
MLA Song, Xiaorui,et al."The immunological capacity in the larvae of Pacific oyster Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 49(2016):461-469.
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