| De novo transcriptome sequencing and analysis of Rapana venosa from six different developmental stages using Hi-seq 2500 | |
Song, Hao1,2; Yu, Zheng-Lin1,2; Sun, Li-Na1; Gao, Yan3 ; Zhang, Tao1; Wang, Hai-Yan1
| |
| 2016-03-01 | |
| 发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
 |
| 卷号 | 17期号:2016页码:48-57 |
| 文章类型 | Article |
| 摘要 | The carnivorous whelk Rapana venosa is regarded as a biological invader with strong ecological fitness in the United States, Argentina, France and other countries. R. venosa may seriously damage bivalve resources. Nonetheless, in China, R. venosa is an important commercial species. Larval development, especially metamorphosis, influences the natural population and industrial breeding. However, there are few studies on the early development of R. venosa, and our understanding is further limited by a lack of genomic information. In this study, de novo sequencing was performed to obtain a comprehensive transcriptome profile during early development. A Hi-seq 2500 sequencing run produced 148,737,902 raw reads that were assembled into 1,137,556 unigenes (average length of 619 nucleotides, of which 49,673 could be annotated). The unigenes were assigned to biological processes and functions after annotation in Gene Ontology, eukaryotic Ortholog Groups and Kyoto Encyclopedia of Genes and Genomes. We also identified 93,196 simple sequence repeats among the unigenes. Six unique sequences associated with neuroendocrine function were analyzed by quantitative real-time PCR. Our data represent the first comprehensive transcriptomic resource for R. venosa. Functional annotation of the unigenes involved in various biological processes could stimulate research on the mechanisms of early development in this species. Understanding the mechanism of early development and metamorphosis would benefit antifouling research and aquaculture of R. venosa. (C) 2016 Published by Elsevier Inc. |
| 关键词 | Transcriptome Rapana Venosa Gastropod Larva |
| DOI | 10.1016/j.cbd.2016.01.006 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000370894500005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/112483 |
| 专题 | 海洋生物分类与系统演化实验室 海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Tianjin Bohai Sea Fisheries Res Inst, Tianjin 300457, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Song, Hao,Yu, Zheng-Lin,Sun, Li-Na,et al. De novo transcriptome sequencing and analysis of Rapana venosa from six different developmental stages using Hi-seq 2500[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2016,17(2016):48-57. |
| APA | Song, Hao,Yu, Zheng-Lin,Sun, Li-Na,Gao, Yan,Zhang, Tao,&Wang, Hai-Yan.(2016).De novo transcriptome sequencing and analysis of Rapana venosa from six different developmental stages using Hi-seq 2500.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,17(2016),48-57. |
| MLA | Song, Hao,et al."De novo transcriptome sequencing and analysis of Rapana venosa from six different developmental stages using Hi-seq 2500".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 17.2016(2016):48-57. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| De novo transcriptom（1103KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论