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Lysozyme as sensitive reporter for fluorometric and PCR based detection of E-coli and S-aureus using magnetic microbeads
Zeng, Yan1,2; Wan, Yi1; Zhang, Dun1
2016-02-01
发表期刊MICROCHIMICA ACTA
卷号183期号:2页码:741-748
文章类型Article
摘要We describe the use of lysozyme as a sensitive reporter for detection of bacteria. The assay comprises the following steps: First, specific primers are used for targeting the rDNA of E. coli and S. aureus, respectively, and the polymerase chain reaction is applied to amplify bacterial DNA. Then, the target DNA binds to the capture probe immobilized on magnetic beads, with lysozyme acting as signal reporter that catalyzes the hydrolysis of an enzyme substrate to form the blue fluorescent product 4-methylumbelliferone. Coupled with multiple lysozyme substrates, this fluorometric detecting method has a detection limit of 50 CFUa <...mL(-1) bacteria. The optical changes may also be detected by colorimetry and monitored with bare eyes. The method was selective over other bacteria and was successfully applied to the determination of E. coli and S. aureus in human serum, which provides a perspective for lysozyme application and species-specific pathogenic bacteria detection.
关键词Bacterial Detection Fluorescence Colorimetry Bare Eye Detection Polymerase Chain Reaction Magnetic Beads
DOI10.1007/s00604-015-1715-1
收录类别SCI
语种英语
WOS记录号WOS:000370176500028
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/112471
专题海洋腐蚀与防护研究发展中心
作者单位1.Chinese Acad Sci, Key Lab Marine Environm Corros & Biofouling, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China
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Zeng, Yan,Wan, Yi,Zhang, Dun. Lysozyme as sensitive reporter for fluorometric and PCR based detection of E-coli and S-aureus using magnetic microbeads[J]. MICROCHIMICA ACTA,2016,183(2):741-748.
APA Zeng, Yan,Wan, Yi,&Zhang, Dun.(2016).Lysozyme as sensitive reporter for fluorometric and PCR based detection of E-coli and S-aureus using magnetic microbeads.MICROCHIMICA ACTA,183(2),741-748.
MLA Zeng, Yan,et al."Lysozyme as sensitive reporter for fluorometric and PCR based detection of E-coli and S-aureus using magnetic microbeads".MICROCHIMICA ACTA 183.2(2016):741-748.
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