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Lysozyme as sensitive reporter for fluorometric and PCR based detection of E-coli and S-aureus using magnetic microbeads
Zeng, Yan1,2; Wan, Yi1; Zhang, Dun1
2016-02-01
Source PublicationMICROCHIMICA ACTA
Volume183Issue:2Pages:741-748
SubtypeArticle
AbstractWe describe the use of lysozyme as a sensitive reporter for detection of bacteria. The assay comprises the following steps: First, specific primers are used for targeting the rDNA of E. coli and S. aureus, respectively, and the polymerase chain reaction is applied to amplify bacterial DNA. Then, the target DNA binds to the capture probe immobilized on magnetic beads, with lysozyme acting as signal reporter that catalyzes the hydrolysis of an enzyme substrate to form the blue fluorescent product 4-methylumbelliferone. Coupled with multiple lysozyme substrates, this fluorometric detecting method has a detection limit of 50 CFUa <...mL(-1) bacteria. The optical changes may also be detected by colorimetry and monitored with bare eyes. The method was selective over other bacteria and was successfully applied to the determination of E. coli and S. aureus in human serum, which provides a perspective for lysozyme application and species-specific pathogenic bacteria detection.
KeywordBacterial Detection Fluorescence Colorimetry Bare Eye Detection Polymerase Chain Reaction Magnetic Beads
DOI10.1007/s00604-015-1715-1
Indexed BySCI
Language英语
WOS IDWOS:000370176500028
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/112471
Collection海洋环境腐蚀与与生物污损重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Marine Environm Corros & Biofouling, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zeng, Yan,Wan, Yi,Zhang, Dun. Lysozyme as sensitive reporter for fluorometric and PCR based detection of E-coli and S-aureus using magnetic microbeads[J]. MICROCHIMICA ACTA,2016,183(2):741-748.
APA Zeng, Yan,Wan, Yi,&Zhang, Dun.(2016).Lysozyme as sensitive reporter for fluorometric and PCR based detection of E-coli and S-aureus using magnetic microbeads.MICROCHIMICA ACTA,183(2),741-748.
MLA Zeng, Yan,et al."Lysozyme as sensitive reporter for fluorometric and PCR based detection of E-coli and S-aureus using magnetic microbeads".MICROCHIMICA ACTA 183.2(2016):741-748.
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