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The immunosuppressive effects of continuous CpG ODNs stimulation in chinese mitten crab, Eriocheir sinensis
Zhao, D.1; Song, L.2; Liu, R.3; Liang, Z.3; Wang, L.3; Sun, M.3; Zhu, B.1
2016
Source PublicationISJ-INVERTEBRATE SURVIVAL JOURNAL
Volume13Pages:34-43
SubtypeArticle
AbstractCpG oligodeoxynucleotides (CpG ODNs) have been widely used as a novel vaccine adjuvant in mammals due to its immune protection, long effectiveness and safety. In the present study, the long-term immune effects as well as immunosuppression of CpG ODNs was evaluated by comparing the immune parameters of Chinese mitten crab, Eriocheir sinensis after continuous or interval feeding with CpG ODNs-supplement diet for 21 days (designated as CC and CI group, respectively). In the CI group, the mRNA transcripts of EsTolls (EsToll1 and EsToll2) and EsMyD88 (adaptor molecule) in hepatopancreas maintained at a significantly higher level (p < 0.05) compared with the CC group after 7 days and 14 days feeding, while there was no significant difference between them at the 21st day. Moreover, after a significant increase at 7th day, the expression level of EsLITAF mRNA [Lipopolysaccharide-induced tumor necrosis factor (TNF)-alpha] in CC group decreased at 14th-21st day, while that in CI group kept increasing at 14th day, followed by a decrease at 21st day. The TNF-alpha in plasma of CC group was abnormally increased at the 21st day (p < 0.05) in CC group compared with the significant raise at 7th-14th in CI group. Moreover, the phagocytic activity and reactive oxygens (ROS) level of hemocytes in continuous CpG ODNs feeding crabs were significantly lower than those in interval feeding crabs. These results indicated that long-term and continuous CpG ODNs stimulation could reduce the activation of pro-inflammatory cytokine production, hemocyte phagocytosis and ROS generation, displaying immunosuppressive effects on the immune system of crabs.
KeywordEriocheir Sinensis Cpg Odns Feeding Immunosuppressive Effects Toll-like Receptor Pathway Tumor Necrosis Factor-alpha Phagocytosis
Indexed BySCI
Language英语
WOS IDWOS:000370174800006
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Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/112457
Collection实验海洋生物学重点实验室
Affiliation1.Dalian Polytech Univ, 1 Qinggongyuan, Dalian 116034, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Zhao, D.,Song, L.,Liu, R.,et al. The immunosuppressive effects of continuous CpG ODNs stimulation in chinese mitten crab, Eriocheir sinensis[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2016,13:34-43.
APA Zhao, D..,Song, L..,Liu, R..,Liang, Z..,Wang, L..,...&Zhu, B..(2016).The immunosuppressive effects of continuous CpG ODNs stimulation in chinese mitten crab, Eriocheir sinensis.ISJ-INVERTEBRATE SURVIVAL JOURNAL,13,34-43.
MLA Zhao, D.,et al."The immunosuppressive effects of continuous CpG ODNs stimulation in chinese mitten crab, Eriocheir sinensis".ISJ-INVERTEBRATE SURVIVAL JOURNAL 13(2016):34-43.
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