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Functional balance between enzymes in malonyl-CoA pathway for 3-hydroxypropionate biosynthesis
Liu, Changshui1,2; Ding, Yamei3; Zhang, Rubing1; Liu, Huizhou1; Xian, Mo1; Zhao, Guang1
2016-03-01
Source PublicationMETABOLIC ENGINEERING
Volume34Issue:2016Pages:104-111
SubtypeArticle
Abstract3-Hydroxypropionate (3HP) is an important platform chemical, and four 3HP biosynthetic routes were reported, in which the malonyl-CoA pathway has some expected advantages but presented the lowest 3HP yield. Here, we demonstrated that this low yield was caused by a serious functional imbalance between MCR-C and MCR-N proteins, responsible for the two-step reduction of malonyl-CoA to 3HP. Then we minimized the enzyme activity imbalance by directed evolution of rate-limiting enzyme MCR-C and fine tuning of MCR-N expression level. Combined with culture conditions optimization, our engineering approaches increased the 3HP titer 270-fold, from 0.15 g/L to 40.6 g/L, representing the highest 3HP production via malonyl-CoA pathway so far. This study not only significantly improved the 3HP productivity of recombinant Escherichia coli strain, but also proved the importance of metabolic balance in a multistep biosynthetic pathway, which should be always considered in any metabolic engineering study. (C) 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.
Keyword3-hydroxypropionate Malonyl-coa Pathway Malonyl-coa Reductase Enzymatic Activity Imbalance RE.ombinant E. Coli
DOI10.1016/j.ymben.2016.01.001
Indexed BySCI
Language英语
WOS IDWOS:000369520200010
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/112441
Collection海洋环境工程技术研究发展中心
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Liu, Changshui,Ding, Yamei,Zhang, Rubing,et al. Functional balance between enzymes in malonyl-CoA pathway for 3-hydroxypropionate biosynthesis[J]. METABOLIC ENGINEERING,2016,34(2016):104-111.
APA Liu, Changshui,Ding, Yamei,Zhang, Rubing,Liu, Huizhou,Xian, Mo,&Zhao, Guang.(2016).Functional balance between enzymes in malonyl-CoA pathway for 3-hydroxypropionate biosynthesis.METABOLIC ENGINEERING,34(2016),104-111.
MLA Liu, Changshui,et al."Functional balance between enzymes in malonyl-CoA pathway for 3-hydroxypropionate biosynthesis".METABOLIC ENGINEERING 34.2016(2016):104-111.
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